CATCH THE BUZZ
By David O’Brochta
A breakthrough in the efforts to genetically modify honey bees was recently
reported by Christina Schulte and colleagues from Heinrich Heine University in
the Proceedings of the National Academy of Sciences of the United States of
Schulte et al. reported the creation of a honey bee containing a “foreign”
gene — in this case, one that made some of the cells in the bee glow. This is a
first in bee research. These researchers did not establish a colony of
genetically-modified bees; they only showed that genetically-manipulated queens
could produce genetically-modified drones in the lab. It was a proof of concept.
We have known the genome sequence of the honey bee, Apis mellifera,
since 2006. The bee genome helps bee biologists learn how honey bees tick, and
it has already provided insights. The genome is rich in genes associated with
smell, but it has relatively fewer genes associated with taste and immune
functions, reflecting evolutionary adaptations associated with their unique
Using genetic technologies in the laboratory to actually manipulate the bee
genome in living bees will lead to deeper insights, such as how they fight
infections like foulbrood disease or parasites like Varroa mites, as
well as the genetic basis for bee behavior.
Imagine you know a little bit about cars and you want to figure out what
makes them run. A manual is available, but it’s in some kind of code. One
approach would be to take a hammer and, starting with one part at a time, break
things and then see how the “mutated” car functions.
“Oh look, now it doesn’t start — that must be a starter thingy,” you might
“Now all the lights and the radio don’t work — that must be an electrical
And so on. Pretty soon you would know a lot about how the car works and the
role of many of its parts, and the coded manual would make more sense too.
This is pretty much how geneticists might approach the problem of
understanding how bees function. Geneticists would not use a hammer, but they
would use genetic technologies to manipulate the genome of living bees to see
how those alterations affected the organism.
Today there are many technologies that enable scientists to insert genes
into chromosomes. In the case of bees, applying those technologies has proven
very difficult. This is because insect-genome-modification technologies require
physically injecting these technologies (usually bits of DNA) into honey bee
eggs, having the eggs hatch and develop into fertile queens, and then getting
the queens to reproduce. However, bees do not like having their eggs injected.
The key to Schulte et al.’s success was their innovative approaches to
manipulating and controlling bee reproduction and behavior in the laboratory so
they could successfully inject their eggs. They have forged an important path
that others can follow, albeit a challenging one.
Just as the human genome enables human biology to be understood for the
purposes of developing therapeutics and solutions to unwanted conditions, these
results represent the beginning of a similar phase of bee research.
David O’Brochta is the director of the Insect Genetic Technology Research Coordination Network
(IGTRCN) and is a professor in the Department of Entomology and the Institute for Bioscience and
Biotechnology Research at the University of Maryland, College Park. He has
an active research laboratory focused on insect genetics and molecular genetics
with interests in the development of insect genetic technologies and their
application to the study of the physiological genetics of mosquitoes, with
particular interest in their disease-vector capabilities. Professor O’Brochta
teaches at the undergraduate and graduate levels, is the Head of the Institute
for Bioscience and Biotechnology Research’s Insect Transformation Facility, and
he is the editor of the Royal Entomological Society’s journal Insect Molecular Biology.